Fluorinase‐Coupled Base Swaps: Synthesis of [¹⁸F]‐5′‐Deoxy‐5′‐fluorouridines

Abstract

Making F-ases: One-pot fluorination/base-swap biotransformations were developed by coupling the fluorinase enzyme to nucleoside phosphorylases to generate 5′-deoxy-5′-fluoronucleosides (FDAs). These biotransformations are amenable to radiolabeling syntheses starting from [18F]fluoride ion, as exemplified by the synthesis of [18F]-5′-deoxy-5′-fluorouridines (see scheme), and demonstrate a new application of fluorinase as a catalyst for 18FC bond formation. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.