Limitations of Spoligotyping and Variable-Number Tandem-Repeat Typing for Molecular Tracing of Mycobacterium bovis in a High-Diversity Setting

Abstract

This study describes the attempt to trace the first Mycobacterium bovis outbreak in alpacas (Lama pacos) in Spain by spoligotyping and variable-number tandem-repeat (VNTR) analysis. Due to high genotype diversity, no matching source was identified, but local expansion of a clonal group was found and its significance for molecular tracing is discussed.