Photochemical Surface Patterning by the Thiol‐Ene Reaction

Abstract

The photochemical coupling of olefin-capped (bio)molecules to surface-bound thiols can be used to control protein immobilization on length scales of centimeters to sub-micrometers (see the fluorescence microscopy image of a nanopattern after treatment with labeled streptavidin). Two enzymes immobilized on the resulting patterns retained their enzymatic activity and underwent protein–protein interactions similar to those in the solution phase. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z800101_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.