Journal of Clinical Microbiology · 2009 · 23 citations · 13 references
Veterinary MedicineInfectious DiseasesMicrobiologyDiagnostic MicrobiologyMolecular VirologyVirologyCentral Nervous System InfectionsDiagnosticsInfectious Disease Molecular TestingPathogen DetectionViral DiagnosticsCerebrospinal Fluid AnalysisCerebrospinal Fluid DiagnosisCerebrospinal Fluid DiagnosticsMultiplex Assay DesignMultiplex Assay DevelopmentMultiplex DiagnosticsMultiplex Ligation-dependent Probe AmplificationPcr-based Detection
A multiplex ligation-dependent probe amplification assay for simultaneous detection of six virus species was developed and tested on clinical cerebrospinal fluid (CSF) samples. The assay, termed MeningoFinder, showed an accordance of 97%, concordance of 96%, interlaboratory sensitivity of 90%, and interlaboratory specificity of 94% compared to PCRs.
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