Infection and Immunity · 1992 · 22 citations · 24 references
Veterinary MedicineMicrobiologyVeterinary ParasitologyParasite ImmunologyParasitologyVeterinary Parasitic DiseasesParasite AntigensParasitic Disease PathogenesisParasitic ZoonosesProtozoal InfectionsProtozoan ImmunologyProtozoan ZoonosesImmunofluorescence AssaysIndirect Immunofluorescence AssayParasite Antibody DetectionParasite-associated Antigen RecognitionParasite-associated AntigensParasite-specific AntibodiesPolyclonal AntibodiesWestern Blot Analysis
Polyclonal antibodies raised against Cryptosporidium parvum oocysts were found to cross-react with Eimeria spp. oocyst antigens in an indirect immunofluorescence assay, and sera from Eimeria spp.-infected lambs reacted with some antigens from sonicated C. parvum oocysts (between 29 to 30 and 66 to 69 kDa) by Western blot (immunoblot). No cross-reaction was observed with cystozoites of Toxoplasma and Sarcocystis spp. These results show the existence of epitopes common to C. parvum and various Eimeria spp.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations