Detection of Carbofuran with Immobilized Acetylcholinesterase Based on Carbon Nanotubes‐Chitosan Modified Electrode

Abstract

A sensitive and stable enzyme biosensor based on efficient immobilization of acetylcholinesterase (AChE) to MWNTs‐modified glassy carbon electrode (GCE) with chitosan (CS) by layer‐by‐layer (LBL) technique for rapid determination of carbofuran has been devised. According to the inhibitory effect of carbamate pesticide on the enzymatic activity of AChE, we use carbofuran as a model pesticide. The inhibitory effect of carbofuran on the biosensor was proportional to concentration of carbofuran in the range from 10 −10 g/L to 10 −3 g/L with a detection limit of 10 −12 g/L. This biosensor is a promising new method for pesticide analysis.