Regeneration of Glomerular Podocytes by Human Renal Progenitors

TLDR

Podocyte loss contributes to glomerulosclerosis, yet it is unclear whether adult podocyte injury can be repaired. We identified a hierarchical CD133⁺CD24⁺ progenitor population in adult human kidneys that can regenerate podocytes and tubular cells, and transplantation of CD133⁺CD24⁺PDX⁻ cells into nephropathy mice reduced proteinuria and improved glomerular injury, indicating therapeutic potential for podocyte repair.

Abstract

Depletion of podocytes, common to glomerular diseases in general, plays a role in the pathogenesis of glomerulosclerosis. Whether podocyte injury in adulthood can be repaired has not been established. Here, we demonstrate that in the adult human kidney, CD133+CD24+ cells consist of a hierarchical population of progenitors that are arranged in a precise sequence within Bowman's capsule and exhibit heterogeneous potential for differentiation and regeneration. Cells localized to the urinary pole that expressed CD133 and CD24, but not podocyte markers (CD133+CD24+PDX− cells), could regenerate both tubular cells and podocytes. In contrast, cells localized between the urinary pole and vascular pole that expressed both progenitor and podocytes markers (CD133+CD24+PDX+) could regenerate only podocytes. Finally, cells localized to the vascular pole did not exhibit progenitor markers, but displayed phenotypic features of differentiated podocytes (CD133−CD24−PDX+ cells). Injection of CD133+CD24+PDX− cells, but not CD133+CD24+PDX+ or CD133-CD24− cells, into mice with adriamycin-induced nephropathy reduced proteinuria and improved chronic glomerular damage, suggesting that CD133+CD24+PDX− cells could potentially treat glomerular disorders characterized by podocyte injury, proteinuria, and progressive glomerulosclerosis.