A Graphene‐Based Platform for the Assay of Duplex‐DNA Unwinding by Helicase

Abstract

Time to unwind: Graphene oxide (GO) enables the quantitative measurement of helicase-dependent double-stranded DNA (dsDNA) unwinding activity in real time. GO selectively binds to unwound fluorescent-dye-labeled single-stranded DNA and quenches its fluorescence (see picture). The helicase activity is monitored by following the change in fluorescence. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.