Environment‐Sensitive Two‐Photon Probe for Intracellular Free Magnesium Ions in Live Tissue

Abstract

“Green” emission: A novel two-photon fluorescent probe that can detect intracellular free Mg2+ ions at a depth of a few hundred micrometers in live tissue was developed. The emission from the Mg2+–probe complex (green) shows little interference from association of the probe with other intracellular metal ions or with the cell membrane (blue).