Doubled haploid plant production of transgenic rice (Oryza sativa L.) using anther culture

Abstract

Anthers of transgenic rice plants with two foreign genes were cultured to obtain doubled haploid plants that possessed the introduced foreign genes homozygously. Anther culture response varied with the transgenic line. Green plantlets were regenerated from the microspore-derived calli, and 5.9–88.9% of the regenerated plants set numerous seeds in a greenhouse. The haploid or doubled haploid green plants were confirmed to have the foreign genes by Southern hybridization analysis. They had both or no foreign genes, and no plants with heterozygosity in transgenes were observed. The ratio of the plants with the transgenes homozygously to those without the transgenes varied with the donor transgenic lines.