American Journal of Tropical Medicine and Hygiene · 2005 · 36 citations · 14 references
Veterinary MedicineGeneticsInfectious DiseasesHost-pathogen InteractionsParasite EpidemiologyParasite ImmunologyBlood Parasite DiagnosisMalariaMolecular Diagnosis Of ParasitesMolecular TypingPathogen DetectionAntimalarial AgentsDrug Efficacy EvaluationDrug Efficacy TestingMalaria SusceptibilityMicrosatellite MarkersMicrosatellite TypingMicrosatellite VariationPlasmodium Falciparum
In vivo tests for susceptibility to antimalarial drugs require molecular methods to distinguish recrudescence from new infection. The most commonly used DNA markers (merozoite surface proteins [MSPs]) are under immune selective pressure, which might lead to misclassification. We evaluated immunologically neutral microsatellite markers in blood samples collected during a drug efficacy trial in Rwanda. Fifty percent of the infections classified as recrudescent by MSP were classified as new by microsatellite markers. Reciprocally, 23.3% of infections classified as recrudescent by microsatellite markers were identified as new by MSP. In drug efficacy studies, microsatellite markers should complement MSP genotyping to distinguish a recrudescence from a new infection.
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Mutation of human short tandem repeats
J. L. Weber, Carmen Chak‐Lui Wong · Human Molecular Genetics · 1993 · 1.6K citations
Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum
John C. Wootton, Xiaorong Feng, Michael T. Ferdig et al. · Nature · 2002 · 728 citations