Oriental Pharmacy and Experimental Medicine · 2014 · 17 citations · 17 references
Life SciencesCell BiologyImmunologyImmunoregulationInflammationInnate ImmunityCytokine BiologyCytokine RegulationCytokine SignalingInflammatory Cell ResponsesInflammatory SignalingMacrophage BiologyAnti-inflammatory ActivityAnti-inflammatory MechanismsCordyceps SpeciesImmune System ModulationInflammatory Cytokine SecretionMacrophage Inflammatory ResponsesPro-inflammatory Cytokine ExpressionTraditional Chinese Medicine
spp. The anti-inflammatory effects of CBG-CS-2 were investigated. The water-soluble fraction of CBG-CS-2 has high anti-inflammatory activity in LPS-induced Raw264.7 macrophages. We tested the role of CBG-CS-2 on the anti-inflammation cascade in LPS-stimulated Raw264.7 cells. CBG-CS-2 significantly decreased NO production, iNOS expression, and pro-inflammatory cytokine secretion in a dose-dependent manner. To investigate the mechanism by which CBG-CS-2 inhibits NO, iNOS, and pro-inflammatory cytokines, we examined the activities of NF-κB and AP-1 in LPS-activated macrophages. The results demonstrate that CBG-CS-2 suppresses the production and expression of NO, iNOS, and pro-inflammatory cytokines in LPS-activated macrophages via inhibition of NF-κB and AP-1, which may play an important role in inflammation. These findings suggest that CBG-CS-2 has modulatory effects on the inflammatory system in macrophages, and that it can serve as a useful anti-inflammatory dietary supplement or drug.
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