A new protocol for extraction of C0t-1 DNA from rice

Abstract

C0t-1 DNA, enriched for repetitive DNA sequences, has been proved to be valuable in the studies of plant species differentiation and genome evolution. A new protocol to steadily obtain the aimed range of DNA fragments has been developed by shearing the genomic DNA with the digest system containing DNase and DNA polymerase . Comparison through fluorescence in situ hybridization (FISH)- Oryza sativa L with labeled probe of C0t-1 DNA isolated by the autoclave equipment and the new procedure was carried out. FISH result shows that the new procedure can be a feasible method to steadily isolate high-quality C0t-1 DNA. Key words: C0t-1 DNA, Deoxyribonuclease , DNA polymerase , rice, repetitive DNA sequence.