Isolation and characterization of a Bacillus strain capable of degrading the extracellular glucan from Cellulomonas flavigena strain KU

Abstract

Bacteria capable of utilizing the water-insoluble purified extracellular (1-->3)-beta-D-glucan (curdlan) from Cellulomonas flavigena strain KU by extracellular enzymes, were isolated and characterized. Enrichment cultures from a Winogradsky column were incubated anaerobically at 55 degrees C with curdlan as the sole source of carbon. Colonies surrounded by zones of clearing were selected from subcultures on solid curdlan media. One of the isolates was chosen for further study and identified by conventional methods, API-tests with calculation of similarity coefficients and ID-scores, estimation of mol% (G+C) and DNA-DNA liquid hybridization. The isolate is a facultatively anaerobic, facultatively thermophilic Bacillus sp. Identification at the species-level was not achieved. The isolate was characterized by some rare traits among bacilli, but it remains unresolved whether it defines a new taxon.