Proceedings of the National Academy of Sciences · 1994 · 537 citations · 27 references
Natural SciencesEnvironmental Social ScienceClimate Change AdaptationClimate ScienceSustainability ScienceAdaptive CapacityClimate AdaptationEnvironmental Systems ModelingHuman Adaptation To ClimateClimate Change Adaptation PolicyDecision Support System ModelsMacro SystemsPolicy ModelsRas Gtpase SignalingRepresentative Agent Models
The nuclear Ras-related protein Ran binds guanine nucleotide and is involved in cell cycle regulation. Models of the signal pathway predict Ran to be active as Ran.GTP at the initiation of S phase upon activation by the nucleotide exchange factor RCC1 and to be inactivated for the onset of mitosis by hydrolysis of bound GTP. Here a nuclear homodimeric 65-kDa protein, RanGAP1, is described, which we believe to be the immediate antagonist of RCC1. It was purified from HeLa cell lysates and induces GTPase activity of Ran, but not Ras, by more than 3 orders of magnitude. The Ran mutant Q69L, modeled after RasQ61L, which is unable to hydrolyze bound GTP, is insensitive to RanGAP1.
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Association between GTPase activators for Rho and Ras families
Jeffrey Settleman, Charles F. Albright, Lauren C. Foster et al. · Nature · 1992 · 319 citations