A Selenium Analogue of Firefly <scp>D</scp> ‐Luciferin with Red‐Shifted Bioluminescence Emission

Abstract

A selenium analogue of amino-D-luciferin, aminoseleno-D-luciferin, is synthesized and shown to be a competent substrate for the firefly luciferase enzyme. It has a red-shifted bioluminescence emission maximum at 600 nm and is suitable for bioluminescence imaging studies in living subjects.