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A TRICHLORACETIC ACID REAGENT FOR THE DETECTION OF PREGNANE-3α:17α:20-TRIOLS ON PAPER CHROMATOGRAMS
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1956
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GynecologySimilar FluorescenceTrichloracetic Acid ReagentMedicinal ChemistryAdrenal GlandSummary 1Gas ChromatographyBioanalysisBlue Fluorescent SpotAnalytical ChemistryLiquid ChromatographyClinical ChemistrySteroid MetabolismChromatographyBiochemistryAdrenal DiseaseChromatographic AnalysisPharmacologyNatural SciencesMedicineDrug Analysis
SUMMARY 1. Pregnane-3α:17α:20-triols give a blue fluorescent spot after heating a chromatogram sprayed with approximately 25% trichloracetic acid in ethanol. A spot containing 2 μg may be detected by this reaction. Out of forty-eight steroids examined only atiocholanediol gave a similar fluorescence. Certain other steroids give different characteristic colours and fluorescence. 2. The test has been successfully used to detect pregnanetriol in the non-ketonic fraction of urine extracts from a normal male and from a patient with adrenal hyperplasia. It has been used to detect the production of pregnanetriol by in vitro reduction of pregnane-3α:17α-diol-20-one. 3. The identity of the substances which gave this reaction and which were obtained from urine and from in vitro reduction was confirmed by isolation and characterization.