Glaciecola arctica sp. nov., isolated from Arctic marine sediment

Abstract

A Gram-negative, motile, psychrotolerant, oxidase- and catalase-positive bacterium, designated BSs20135 T , was isolated from Arctic marine sediment. Cells were straight or slightly curved rods and formed circular, convex and yellowish-brown colonies. Buds and prosthecae could be produced. The strain grew at 4–28 °C (optimum 25 °C) and with 1–5 % (w/v) NaCl (optimum 2 %) and hydrolysed aesculin and DNA, but did not reduce nitrate to nitrite. Phylogenetic analysis of 16S rRNA gene sequences indicated that strain BSs20135 T belonged to the genus Glaciecola and shared 93.6–97.7 % sequence similarity with the type strains of known species of the genus Glaciecola . The major cellular fatty acids of strain BSs20135 T were summed feature 3 (comprising C 16 : 1 ω7 c and/or iso-C 15 : 0 2-OH), C 16 : 0 , C 17 : 1 ω8 c and C 18 : 1 ω7 c . The genomic DNA G+C content was 40.3 mol%. Based on 16S rRNA gene sequence analysis, DNA–DNA hybridization data and phenotypic and chemotaxonomic characterization, strain BSs20135 T represents a novel species, for which the name Glaciecola arctica sp. nov. is proposed. The type strain is BSs20135 T ( = CCTCC AB 209161 T = KACC 14537 T ).