Science · 2005 · 1.1K citations · 25 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationBacterial Gene RegulationGene Expression DynamicsGene Expression RegulationGenetic Circuit DesignSynthetic Gene CircuitsTranscriptional Regulation In ProkaryotesFluorescent Reporter SystemsGene Regulatory NoiseIntrinsic NoiseProtein ProductionReporter GenesSynthetic Gene Circuit DesignTranscriptional Regulation CircuitsTranscriptional Regulation Modeling
The quantitative relation between transcription factor concentrations and the rate of protein production from downstream genes is central to the function of genetic networks. Here we show that this relation, which we call the gene regulation function (GRF), fluctuates dynamically in individual living cells, thereby limiting the accuracy with which transcriptional genetic circuits can transfer signals. Using fluorescent reporter genes and fusion proteins, we characterized the bacteriophage lambda promoter P(R) in Escherichia coli and calibrated in vivo biochemical parameters in molecular units via a novel technique based on binomial errors in protein partitioning. Protein production rates fluctuate over a cell‑cycle timescale while intrinsic noise decays rapidly, and together with biochemical parameters and slowly varying cellular states, these factors determine the effective single‑cell GRF, providing a basis for quantitative modeling of natural gene circuits and design of synthetic ones.
The quantitative relation between transcription factor concentrations and the rate of protein production from downstream genes is central to the function of genetic networks. Here we show that this relation, which we call the gene regulation function (GRF), fluctuates dynamically in individual living cells, thereby limiting the accuracy with which transcriptional genetic circuits can transfer signals. Using fluorescent reporter genes and fusion proteins, we characterized the bacteriophage lambda promoter P(R) in Escherichia coli. A novel technique based on binomial errors in protein partitioning enabled calibration of in vivo biochemical parameters in molecular units. We found that protein production rates fluctuate over a time scale of about one cell cycle, while intrinsic noise decays rapidly. Thus, biochemical parameters, noise, and slowly varying cellular states together determine the effective single-cell GRF. These results can form a basis for quantitative modeling of natural gene circuits and for design of synthetic ones.
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Stochastic Gene Expression in a Single Cell
Michael B. Elowitz, Arnold J. Levine, Eric D. Siggia et al. · Science · 2002 · 5.7K citations
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