Cell Transformation: A Study of Regulation with Types 5 and 12 Adenovirus Temperature-sensitive Mutants

Abstract

Since the initial isolations of adenoviruses from man (Rowe et al. 1953; Hilleman and Werner 1954), these viruses have served as models for studies of productive (Green 1966; Ginsberg 1969; Schlesinger 1969) and latent infections (Rowe et al. 1953; Strohl and Schlesinger 1965), as well as for the investigation of viral oncogenesis (Trentin et al. 1962) and cell transformation (Pope and Rowe 1964). Extensive biological and biochemical data have been obtained describing this wide array of cellular responses induced by adenoviruses. Yet these studies gave only limited information for a complete understanding of the molecular reactions that regulate viral replication and the pathological consequences of the virus-host cell encounter. It became apparent that viral, and probably host cell, mutants would be needed for further investigation of the precise mechanisms regulating the biochemical events in viral synthesis and cell alterations. Accordingly, several laboratories have isolated a valuable variety of conditionally lethal...