A New extractive spectrophotometric method for determination of rizatriptan dosage forms using bromocresol green

Abstract

BACKGROUND AND THE PURPOSE OF THE STUDY: Rizatriptan is used effectively for the treatment of migraine headache. In this study, a simple, rapid and low cost spectrophotometric method based on the ion-pair complexation is proposed for the determination of rizatriptan in raw material and dosage forms. METHODS: The ion-pair complexation using bromocresol green as reagent was performed in a buffer solution and the absorbance was measured by a spectrophotometer. The ion-pair formation conditions were optimized and the accuracy and precision of the method were calculated. RESULTS AND MAJOR CONCLUSION: Best results were achieved by using 6 ml of the bromocresol green reagent in the presence of phosphate buffer (pH 3.0). The stoichiometry of the resulted complex was 1:1. The within-day and between-day precision values were lower than 2.9 and 1.8 percent for the calibration range of 0.5-50 and 10-100 μg/ml, respectively. The proposed method was successfully used for the determination of rizatriptan in dosage forms without any interference.