Graphene‐Oxide‐Based Immunosensing through Fluorescence Quenching by Peroxidase‐Catalyzed Polymerization

Abstract

A graphene oxide (GO)-based immunosensor is developed for the detection of interleukin-5 (IL-5), a key cytokine associated with asthma pathology and eosinophilia. The immunosensing platform utilizes the innate fluorescence of GO, not demanding biomolecules labeled with fluorescent dyes. The GO-based immunoassay exhibits high specificity for IL-5 among other cytokines and is not affected by nonspecific proteins in human serum.