Glucose Biosensors Based on Carbon Nanotube Nanoelectrode Ensembles

TLDR

The study develops glucose biosensors using carbon nanotube nanoelectrode ensembles for selective glucose detection. Glucose oxidase is covalently attached to CNT NEEs via carbodiimide chemistry, and the enzymatic oxidation of glucose produces hydrogen peroxide that is catalytically reduced at the CNT tips, enabling selective electrochemical detection. The resulting biosensor selectively measures glucose amid common interferents such as acetaminophen, uric acid, and ascorbic acid, eliminating the need for permselective membranes or artificial mediators and simplifying sensor design.

Abstract

This paper describes the development of glucose biosensors based on carbon nanotube (CNT) nanoelectrode ensembles (NEEs) for the selective detection of glucose. Glucose oxidase was covalently immobilized on CNT NEEs via carbodiimide chemistry by forming amide linkages between their amine residues and carboxylic acid groups on the CNT tips. The catalytic reduction of hydrogen peroxide liberated from the enzymatic reaction of glucose oxidase upon the glucose and oxygen on CNT NEEs leads to the selective detection of glucose. The biosensor effectively performs a selective electrochemical analysis of glucose in the presence of common interferents (e.g., acetaminophen, uric and ascorbic acids), avoiding the generation of an overlapping signal from such interferers. Such an operation eliminates the need for permselective membrane barriers or artificial electron mediators, thus greatly simplifying the sensor design and fabrication.