Antimicrobial Agents and Chemotherapy · 2011 · 210 citations · 23 references
GeneticsMolecular BiologyTransposon InvolvedGene CharacterizationMolecular GeneticsGenomicsTranscriptional RegulationTranslational BiologyGene StructureGenome EngineeringActive TransposonCarbapenemase Gene BlaMolecular MicrobiologyGene ExpressionFunctional GenomicsCell BiologyTranscription RegulationGene FunctionTransposition-conjugation AssayNatural SciencesGene RegulationFunctional CharacterizationMicrobiologyTranscription FactorsMedicineMicrobial Genetics
The carbapenemase gene bla(KPC), which is rapidly spreading worldwide, is located on a Tn3-based transposon, Tn4401. In a transposition-conjugation assay, Tn4401 was able to mobilize bla(KPC-2) gene at a frequency of 4.4 × 10(-6)/recipient cell. A 5-bp target site duplication was evidenced upon each insertion without target site specificity. This study demonstrated that Tn4401 is an active transposon capable of mobilizing bla(KPC) genes at high frequency.
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María Virginia Villegas, Karen Lolans, Adriana Correa et al. · Antimicrobial Agents and Chemotherapy · 2007 · 292 citations · Full text
First Identification, Bacteriology, Antibiotic Resistance +17