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Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping

Marcelo Miyata, Adolfo Carlos Barreto Santos, Natália Helena Mendes, Eunice Atsuko Totumi Cunha, Fernando Augusto Fiúza de Melo, Clarice Queico Fujimura Leite

Brazilian Journal of Microbiology · 2011 · 13 citations · 11 references

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Abstract

We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping.

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