Diafiltration MALDI-TOF Mass Spectrometry Method for Culture-Independent Detection and Identification of Pathogens Directly From Urine Specimens

Abstract

Using the diafiltration method, we were able to improve the turnaround time for microorganism identification from 24 to 48 hours (for conventional culture) to 2 to 3 hours. Although methodological refinements are under way to further improve the clinical sensitivity and turnaround time, the 100% positive predictive value of this method suggests that it could be used to guide the selection of antimicrobial agents.