Multiplex Detection of Mutations in Clinical Isolates of Rifampin-Resistant <i>Mycobacterium tuberculosis</i> by Short Oligonucleotide Ligation Assay on DNA Chips

Abstract

A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates were analyzed, resulting in 87.3% accuracy and 83.6% concordance relative to DNA sequencing.