Click‐Mediated Labeling of Bacterial Membranes through Metabolic Modification of the Lipopolysaccharide Inner Core

Abstract

Clicking bugs: Metabolically active Gram-negative bacteria can specifically incorporate an azide-modified analogue of 3-deoxy-D-manno-octulosonic acid (1; see scheme) into the lipopolysaccharides on their membranes. This process adds a bioorthogonal chemical reporter onto the cell surface and allows the rapid fluorescent labeling of viable cells by click chemistry. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.