Selective and efficient mitochondrial staining with designed 2,1,3-benzothiadiazole derivatives as live cell fluorescence imaging probes - Concepedia

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Selective and efficient mitochondrial staining with designed 2,1,3-benzothiadiazole derivatives as live cell fluorescence imaging probes

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2012

Year

Brenno A. D. Neto, José R. Corrêa, Pedro H. P. R. Carvalho, Diego C. B. D. Santos, Bruna C. Guido, Claudia C. Gatto, Heibbe C. B. de Oliveira, Maíra Fasciotti, Marcos N. Eberlin, Eufrânio N. da Silva Júnior

Journal of the Brazilian Chemical Society

EngineeringOncologic Imaging2,1,3-Benzothiadiazole DerivativesLive Cell Fluorescence2,1,3-Benzothiadiazole Fluorescent ProbesEfficient Mitochondrial StainingTranslational Molecular ImagingMolecular ImagingNovel Imaging MethodNew DyesBiochemistryFluorescence ImagingCell BiologySingle-molecule DetectionBiomolecular EngineeringMitochondrial FunctionCellular BiochemistryChemical ProbeMedicineCell Imaging

Abstract

Novel designed 2,1,3-benzothiadiazole fluorescent probes were synthesized, characterized and applied as live cell fluorescence imaging probe staining only mitochondria in mammalian cancer cell lines (MCF-7). The efficiency of these new probes was found to be much superior to that of the commercially available MitoTracker® Red. Cellular and in vitro experiments allowed better understanding of the relationship between the planned molecular architecture of the new dyes and the observed cellular selectivity.