Auxin‐stimulated RNA Synthesis in Oat Coleoptile Cells

Abstract

Abstract Using oat coleoptile segments the following results were obtained. Ten mg/l auxin (indole‐3‐acetic acid) increased the incorporation of uracil‐2‐ 14 C and orthophosphate‐ 32 P into RNA fraction during a relatively short incubation period. Stimulation of 32 P incorporation due to auxin was found only in the region heavier than ribosomal RNA, probably in the messenger RNA region. The stimulation of uracil‐2‐ 14 C incorporation into RNA caused by auxin was not influenced by the presence of 0.3 M mannitol which prevents osmotically the water absorption of cells. It is concluded that auxin primarily stimulates the biosynthesis of RNA, possibly messenger, in oat coleoptile cells.