The Nonsynchronous Flow-Through Coil Planet Centrifuge without Rotating Seals Applied to Cell Separation

Abstract

Abstract A new nonsynchronous flow-through coil planet centrifuge allows continuous elution through a coiled separation column without the use of rotating seals. Rates of rotation and revolution of the column are independently adjustable to meet the requirements for the separation of cell particles. The capability of the present apparatus was demonstrated by the separation of human and sheep erythrocytes using an isotonic saline solution.