Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus <i>Puccinia triticina</i>

Abstract

Abstract We report the characterization of 12 polymorphic microsatellite markers in the biotrophic fungus Puccinia triticina , the causal agent of leaf rust on wheat. An enrichment protocol was used to isolate microsatellite loci and polymorphism was explored with 15 European isolates. Significant level of cross‐amplification (44% of the loci) was found in P. striiformis .