Production and Release of Acetylcholinesterase by a Primary Cell Culture of Bovine Adrenal Medullary Chromaffin Cells

Abstract

Bovine adrenal chromaffin cells were isolated and maintained as primary culture monolayers. Total acetylcholinesterase (AChE) activity in the cells increased during the culture period, and AChE activity appeared in the culture medium. We have examined the role of the AChE synthesized by the cells on ACh-evoked release of catecholamine from the cells. A progressive decrease in the efficacy of ACh (5 X 10(-5) M) to evoke release of [3H]norepinephrine from day 3-15 cultures suggests that exogenously applied ACh is hydrolyzed by the nascent AChE synthesized by the cells. These findings provide evidence that chromaffin cells produce AChE and release it into their immediate environment.