(IscS‐IscU)₂ Complex Structures Provide Insights into Fe₂S₂ Biogenesis and Transfer

Abstract

The center of attention: IscS cysteine desulfurases and IscU scaffolds are involved in biological iron–sulfur cluster assembly. The X-ray structure of an anaerobically produced, mutated (Fe2S2-(IscS-IscUD35A))2 complex reveals a cluster coordinated by three IscU cysteines and the IscS active cysteine (see picture). In air-exposed crystals the cluster is oxidized to an Fe2S–S center; D35 is essential for complex dissociation. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.