Kinetic and antigenic similarities for cellobiose dehydrogenase from the brown rot fungus<i>Coniophora puteana</i>and the white rot fungus<i>Phanerochaete chrysosporium</i>

Abstract

Cellobiose dehydrogenase was purified from the brown rot fungus Coniophora puteana. Strong cross-reaction was observed with antibodies to cellobiose:quinone oxidoreductase from the white rot fungus Phanerochaete chrysosporium. Kinetic measurements were made with cellobiose as electron donor. Ferricyanide and DCPIP both showed a pH optimum close to pH 4, but activity with ferricyanide declined more rapidly when the pH was raised. Dioxygen reduction to hydrogen peroxide was observed, but at a much lower rate than for other acceptors. These properties are similar to those of cellobiose dehydrogenase from P. chrysosporium, despite differences between brown and white rot modes of decay.