In Vitro Biosynthesis of the Prepeptide of Type‐III Lantibiotic Labyrinthopeptin A2 Including Formation of a CC Bond as a Post‐Translational Modification

Abstract

A way through the labyrinth: LabKC is an enzyme encoded in the biosynthesis gene cluster (lab gene cluster) of the labyrinthopeptin producer Actinomadura namibiensis. Some of its genes are homologous with those in other actinomycetes strains, for example, the model organism Streptomyces coelicolor. The functional assignment of LabKC as a kinase–cyclase suggests the formation of a new post-translational modification by a consecutive double Michael addition (see scheme; Dha=α,β-dehydroalanine). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.