Quantitative determination of phyllanthin in Phyllanthus amarus by high- performance thin layer chromatography

Abstract

"A simple, precise and rapid high-performance thin-layer chromatographic method has been developed for the estimation of phyllanthin and is the important lignans of Phyllanthus amarus. Separation of phyllanthin was carried out on silica gel 60 F254 layers eluted with hexane: ethyl acetate (2:1), and the analytes were visualized through colour development with 10% concentrated sulphuric acid in ethanol. Scanning and quantification of spots was performed at 200 nm. The proposed method being precise and sensitive can be used for the detection, monitoring and quantification of phyllanthin from Phyllanthus amarus."