Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP 4

TLDR

Monoclonal antibodies against HSV‑1 were generated by two immunization protocols—one using infectious virus in mouse cells and another using cycloheximide‑treated infected cells—and were characterized by SDS‑PAGE of radioimmunoprecipitates, immunofluorescence, and neutralization assays. The study yielded 52 monoclonal antibodies targeting 10 HSV‑1 proteins, revealing that gD, ICP9, ICP6, and the 68‑kDa protein possess both type‑specific and cross‑reactive epitopes, while all gB antibodies cross‑reacted with HSV‑2 and all gC antibodies were type‑specific.

Abstract

Monoclonal antibodies were prepared against herpes simplex virus type 1 (strain 14012) by two immunization procedures. Procedure A utilized infectious virus propagated in mouse cells, and procedure B utilized mouse cells infected with herpes simplex virus in the presence of cycloheximide and harvested 1 h after removal of the inhibitor. A total of 52 monoclonal antibodies were obtained against 10 herpes simplex virus proteins, including four glycosylated proteins (a 110,000-molecular-weight protein, gB, gC, and gD) and six nonglycosylated proteins (a 68,000-molecular-weight protein, ICP 9, ICP 8, ICP 6, ICP 5, and the immediate-early ICP 4). The antibodies were assayed against herpes simplex virus types 1 and 2 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of radioimmunoprecipitates, immunofluorescence, and neutralization. Using the reagents prepared, we concluded that the 110,000-molecular-weight protein, gD, ICP 9, ICP 9, ICP 6, and the 68,000-molecular-weight protein express both type-specific and cross-reactive antigenic determinants. In contrast, nine antibodies against gB all cross-reacted with herpes simplex virus type 2, whereas eight antibodies to gC all reacted type specifically.