Cold Spring Harbor Symposia on Quantitative Biology · 1979 · 171 citations · 0 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGenetic EngineeringMicrobial GeneticsGene CloningMolecular CloningPlasmid BiologyPlasmid GeneticsRecombinant Plasmid ConstructionYeast GeneticsBacterial Plasmid ConstructionEscherichia ColiPlasmid CloningPlasmid Gene TransferPlasmid TransferPlasmid VectorsYeast Cloning SystemsYeast Plasmid Biology
The ability to introduce DNA molecules into living cells by transformation has played a key role in the development of molecular cloning techniques in bac-teria and, thus, in our understanding of gene structure and the control of gene expression. We have used yeast genes cloned on hybrid bacterial plasmids to develop a transformation system for Saccharomyces cerevisiae (Hinnen et al. 1978). Plasmids such as pYeleulO, an Escherichia coli yeast hybrid containing the LEU2 + gene from yeast inserted into the bacterial plasmid ColE1 (Ratzkin and Carbon 1977) provided a highly enriched source of individual yeast genes and enabled us to verify the transformation event at he molecular level by the identification of the ColE1 sequences in the transformants.