Type x collagen synthesis in human osteoarthritic cartilage. indication of chondrocyte hypertrophy

TLDR

Osteoarthritis is a degenerative joint disease characterized by progressive cartilage breakdown. The study investigates the presence of hypertrophic chondrocytes in osteoarthritic cartilage using type X collagen as a specific marker. The authors examined type X collagen biosynthesis by metabolic labeling of isolated chondrocytes with 3‑H.

Abstract

Abstract Objective. To investigate the appearance of hypertrophic chondrocytes in osteoarthritic (OA) cartilage, using type X collagen as a specific marker. Methods. The biosynthesis of type X collagen was examined by metabolic labeling of freshly isolated articular chondrocytes with 3 H‐proline, immunoprecipitation, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis of the synthesized collagens. Extracellular deposition of types X and II collagen was analyzed immunohistochemically. Results. Immunostaining revealed an irregular distribution of type X collagen, which was localized around chondrocyte clusters in fibrillated OA cartilage, but was absent from the noncalcified region of normal articular cartilage. Freshly isolated OA chondrocytes synthesized predominantly type X collagen, while control chondrocytes synthesized mostly type II collagen. Conclusion. Our findings indicate focal premature chondrocyte differentiation to hypertrophic cells in OA cartilage.