Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae

Abstract

A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated.