The Transcriptional Landscape of the Mammalian Genome

TLDR

The study aims to comprehensively map transcription start and termination sites and analyze previously unidentified full‑length cDNAs in the mouse genome. The authors performed comprehensive polling of transcription start and termination sites and analyzed full‑length cDNAs derived from the mouse genome. The authors identified 181,047 transcripts, including 16,247 novel protein‑coding transcripts encoding 5,154 new proteins, revealing transcriptional forests with bidirectional overlapping transcription separated by deserts, and providing a comprehensive platform for comparative analysis of mammalian transcriptional regulation during differentiation and development.

Abstract

This study describes comprehensive polling of transcription start and termination sites and analysis of previously unidentified full-length complementary DNAs derived from the mouse genome. We identify the 5' and 3' boundaries of 181,047 transcripts with extensive variation in transcripts arising from alternative promoter usage, splicing, and polyadenylation. There are 16,247 new mouse protein-coding transcripts, including 5154 encoding previously unidentified proteins. Genomic mapping of the transcriptome reveals transcriptional forests, with overlapping transcription on both strands, separated by deserts in which few transcripts are observed. The data provide a comprehensive platform for the comparative analysis of mammalian transcriptional regulation in differentiation and development.