Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose

Abstract

Abstract Bacteriophage T4, bacteriophage T4 without its tail fibers, and bacteriophage P22 without its tail have been subjected to electrophoresis in agarose gels; electrophoretic mobility (μ) of these particles as a function of the concentration of agarose ( A ) has been determined using preparations of agarose with different amounts of electro‐osmosis. When μ was extrapolated to an A of 0 (μ o ), it was found that a term, μ E , constant for each preparation of agarose must be added to μ o to correct for electro‐osmotic flow. A relationship between μ E and a manufacturer‐determined coefficient of electro‐osmosis has been empirically derived. Corrected μ o 's are in agreement with data obtained by solid support‐free electrophoresis.