Imaging and Quantifying Chemical and Physical Properties of Native Proteins at Molecular Resolution by Force–Volume AFM

Abstract

Use the force: Force–volume atomic force microscopy (AFM) can image native membrane proteins and quantify and map their chemical and physical properties at molecular resolution (see images). For the light-driven proton pump bacteriorhodopsin (BR), the data shows that lipids form a flexible framework embedding a mechanically anisotropic proton pump, and that the BR adopts different structurally stable conformations that are important for proton pumping. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.