THE Si:C:N RATIO OF MARINE DIATOMS: INTERSPECIFIC VARIABILITY AND THE EFFECT OF SOME ENVIRONMENTAL VARIABLES¹

TLDR

The study examined variability of marine diatom Si:C and Si:N ratios to evaluate their usefulness as ecological conversion factors. Across 27 species, mean Si:C and Si:N ratios were 0.13 and 1.05, with little difference between light regimes, higher ratios in larger species, diurnal and light intensity variations up to twofold, and the ratios can convert biogenic silica to nitrogen or carbon within a factor of three. Data are reported in the cited literature.

Abstract

ABSTRACT The variability of marine diatom Si:C and Si:N composition ratios was examined to assess their utility as ecological conversion factors. Twenty‐seven diatom species grown under an 18:6 h LD cycle and sampled at the end of the light period gave mean ratios, by atoms, of 0.13 ± 0.04 and 1.12 ± 0.33 for Si:C and Si:N ratios, respectively (95% C.I. reported). The mean ratios for 18 species grown under continuous illumination were 0.12 ± 0.03 for Si:C and 0.95 ± 0.23 for Si:N. The mean ratios of the clones grown under constant light were not statistically different from those calculated for the same species grown under an 18:6 h LD photoperiod. The overall mean Si:C and Si:N ratios for the 18:6 h LD and continuous light experiments taken together, weighted by the number of species in each experiment, are 0.13 and 1.05, respectively. The average ratios for the nine nanoplankton species (<20 μm) examined were 0.09 ± 0.03 for Si:C and 0.80 ± 0.35 for Si:N. The eighteen netplankton species (>20 μm) had higher mean ratios, Si:C = 0.15 ± 0.04 and Si:N = 1.20 ± 0.37. Time course sampling throughout a 24 h period revealed twofold variations in both ratios for individual species grown on a 14:10 h LD cycle. Changes in irradiance can also produce factor of two variations, both ratios being higher under low light. Comparisons of these data with those from the literature regarding the effects of temperature and nutrient limitation on diatom elemental composition suggest that use of these ratios to convert field estimates of biogenic silica into nitrogen or carbon units, or to estimate silica production from 14 C data, should yield results accurate to within a factor of three under most circumstances.