Observation of Subcellular Metabolite Gradients in Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry

Abstract

Getting to the point: Cell microdissection and analysis by the title technique was used to study the compartmentalization of metabolites within subcellular domains. Experiments performed on plant epidermal cells imply that there are significant differences between the metabolite composition in the nucleus and the cytoplasm. This technique is applicable to other cell types and may give insight into the mechanisms of disease and drug delivery. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.