A quantitative, non-isotopic bioassay for interleukin 2

Abstract

A quantitative bioassay for IL2 is described in which an electronic particle counter is used to measure IL2-mediated proliferation of an IL2-dependent cell line. The assay requires no radioisotopes, is comparable to tritiated thymidine incorporation and can be completely performed in about 28 hours. It can also be used to measure inhibition of IL2-mediated proliferation.