Antimicrobial Agents and Chemotherapy · 2013 · 31 citations · 10 references
MedicineMedicinal ChemistryPharmacologyBiochemical PharmacologyDrug DiscoveryOrganic ChemistryAntiparasitic AgentsAntiparasitic PharmacologyEnzyme InhibitionMarine Drug DiscoveryParasitic ProtozoaProtozoan Host-parasite InteractionsFluorescence-based AssaysFluorescence-based ScreeningImidazole AlkaloidsIndole AlkaloidsMarine AlkaloidsPlant AlkaloidsPlasmodium FalciparumTrophozoite Stage
A library of enriched marine natural product fractions was screened for their antiplasmodial activity using a SYBR green I fluorescence-based assay. Fractions derived from a sponge of the genus Spongosorites exhibited potent inhibition of Plasmodium falciparum growth. This genus of sponge has been reported to contain the nortopsentin and topsentin class of bis-indole imidazole alkaloids. This is the first report of nortopsentin A inhibiting parasite growth at the trophozoite stage at submicromolar 50% inhibitory concentrations (IC(50)).
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