FEMS Microbiology Letters · 2002 · 103 citations · 32 references
Life SciencesMicrobiologyMolecular BiologyBacteriologyMedical MicrobiologyOral MicrobiologyDna Quantification From Dental SamplesMicrobiological AnalysisOral Microbial DiagnosticsOral Microbial GeneticsPathogen DetectionPolymerase Chain ReactionDna Fragment DetectionMethod Detection LimitPcr-based DetectionPolymerase Chain Reaction (Pcr) AssaysReal-time Polymerase Chain Reaction (Pcr)Real-time Polymerase Chain Reaction AssaysStreptococcus SobrinusStreptococcus Species
A real-time polymerase chain reaction (PCR) assay was developed for the quantification of Streptococcus mutans. Primers targeting gtf genes of S. mutans were designed and tested for their specificity using 28 oral streptococcal strains, three other bacterial strains, and human DNA. The primers could amplify specifically the target DNA fragment from a mixture of oral streptococcus genomic DNA containing about 10 fg to 10 ng of S. mutans genome DNA. The real-time PCR produced a linear quantitative detection range over concentrations spanning seven exponential values, with a detection limit of a few copies of S. mutans' genomic DNA per reaction tube. The results of the real-time PCR assay corresponded well to those of conventional culture assays for S. mutans in saliva samples. A real-time PCR assay for Streptococcus sobrinus and Streptococcus downei was also established and produced results that corresponded well to those from conventional culture assays for S. sobrinus in saliva samples. These assays will be useful as a new means to assess one of the important risk factors for caries.
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A selective medium for Streptococcus mutans
Olga Gold, H.V. Jordan, J. van Houte · Archives of Oral Biology · 1973 · 799 citations