Combining Suppression Subtractive Hybridization and Microarrays To Map the Intraspecies Phylogeny of <i>Flavobacterium</i> <i>psychrophilum</i>

Abstract

Reciprocal subtractive libraries were prepared for two strains of Flavobacterium psychrophilum, one virulent and the other avirulent in a trout challenge model. Unique clones were sequenced and their distribution assessed among 34 strains. The analysis showed that F. psychrophilum is composed of two genetic lineages, possibly reflecting host specificity.